| LI Bang-yin,YING Wan-tao,DAI Wei-ming,et al.Down-regulation of methylthioadenosine phosphorylase in malignant BERP35T-2 cells and in non-small cell lung cancers[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):222-225 |
| Down-regulation of methylthioadenosine phosphorylase in malignant BERP35T-2 cells and in non-small cell lung cancers |
| Received:August 28, 2003 |
| DOI： |
| KeyWords:α particles MTAP NSCLC |
| FundProject:国家重点基础研究发展规划973基金资助项目(G1998051207) |
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| Abstract:: |
| Objective The aim of this study was to investigate the expression alteration of MTAP gene in tumorgenesis of bronchial epithelial cell line and Chinese non-small lung cancers. Methods Total protein from BEP2-D and BERP35T-2 cells was extracted and analyzed by 2-D electrophoesis and peptide mass fingerprinting spectrum.Northern blot was performed to confirm differences of expression of MTAP gene between BEP2D and BERP35T-2.RT-PCR was used to observe the mRNA expression of MTAP in 15 clinical cases with non-small cell lung cancer. Results MTAP was found to be expressed at lower level in malignant BERP35T-2 cells than in BEP2D analyzed by 2-dimensional electrophoresis,which was confirmed at transcriptional level by Northern blot.In 11 out of 15 NSCLC samples analyzed by RT-PCR,MTAP expression was down-regulated.MTAP was poorly expressed in 90.9% of mildpoorly differentiated NSCLC cases,while in 25% of well-differentiated cases. Conclusion The result of this study indicates that the down-regulations of MTAP at protein level or mRNA level in Chinese NSCLC might contribute to the carcinogenesis of lung and MTAP might be a candidate of tumor suppressor gene. |
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