LIU Fen-ju,CHEN Jian,LI Bing-yan.Effects of irradiation with 60Co γ-rays on the ratio of DNA and RNA in CNI-H446 cells and its three-dimensional structure by laser scanning confocal microscopy[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):203-204
Effects of irradiation with 60Co γ-rays on the ratio of DNA and RNA in CNI-H446 cells and its three-dimensional structure by laser scanning confocal microscopy
Received:October 16, 2003  
DOI:
KeyWords:Laser scanning confocal microscope (LSCM)  CNI-H446 strain  DNA  RNA  60Co γ radiation
FundProject:苏州大学医学发展基金重大项目资助(2003)
Author NameAffiliation
LIU Fen-ju Department of Medical Radiobiology, School of Radiation Medicine and Public Health, Suzhou University, Nuclear Medicine Key Laboratory, Suzhou 215006, China 
CHEN Jian Department of Medical Radiobiology, School of Radiation Medicine and Public Health, Suzhou University, Nuclear Medicine Key Laboratory, Suzhou 215006, China 
LI Bing-yan Department of Medical Radiobiology, School of Radiation Medicine and Public Health, Suzhou University, Nuclear Medicine Key Laboratory, Suzhou 215006, China 
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Abstract::
      Objective To investigate the effect of γ-radiation on DNA and RNA metabolism in cells of small cell lung cancer (SCLC) strain (CNI-H446) and their three-dimensional structure. Methods Laser scanning confocal microscopy (LSCM) and acridine orange (AO) staining was used to investigate the DNA/RNA changes in the cells,and automatic three-dimensional image scan was used to fine the change of its structure. Results The DNA/RNA of the control group was 1.15±0.07.When the dose of γ-radiation was more than 1 Gy,DNA decreased obviously and the extent of the decrease of DNA was larger than that of RNA.The values of DNA/RNA of the groups that were irradiated with 1 Gy and 3 Gy were significantly different from those of the control group (P<0.01).The three-dimensional image manifested that after large dose of irradiation,the RNA in the cells gathered obviously while DNA were disrupted.When the dose was 8 Gy,the value of DNA/RNA was not different from that of the control group.When the dose was 8 Gy or higher,RNA was suppressed as seriously as DNA,and the value of DNA/RNA increased.There is certain relationship between the value of DNA/RNA and the dose of radiation. Conclusion After irradiated with different dose of γ-radiation,the ratio of DNA and RNA and the size of cells would change correspondingly.
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