LIU Fen-ju,CHEN Jian,LI Bing-yan.Effects of irradiation with 60Co γ-rays on the ratio of DNA and RNA in CNI-H446 cells and its three-dimensional structure by laser scanning confocal microscopy[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):203-204 |
Effects of irradiation with 60Co γ-rays on the ratio of DNA and RNA in CNI-H446 cells and its three-dimensional structure by laser scanning confocal microscopy |
Received:October 16, 2003 |
DOI: |
KeyWords:Laser scanning confocal microscope (LSCM) CNI-H446 strain DNA RNA 60Co γ radiation |
FundProject:苏州大学医学发展基金重大项目资助(2003) |
Author Name | Affiliation | LIU Fen-ju | Department of Medical Radiobiology, School of Radiation Medicine and Public Health, Suzhou University, Nuclear Medicine Key Laboratory, Suzhou 215006, China | CHEN Jian | Department of Medical Radiobiology, School of Radiation Medicine and Public Health, Suzhou University, Nuclear Medicine Key Laboratory, Suzhou 215006, China | LI Bing-yan | Department of Medical Radiobiology, School of Radiation Medicine and Public Health, Suzhou University, Nuclear Medicine Key Laboratory, Suzhou 215006, China |
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Abstract:: |
Objective To investigate the effect of γ-radiation on DNA and RNA metabolism in cells of small cell lung cancer (SCLC) strain (CNI-H446) and their three-dimensional structure. Methods Laser scanning confocal microscopy (LSCM) and acridine orange (AO) staining was used to investigate the DNA/RNA changes in the cells,and automatic three-dimensional image scan was used to fine the change of its structure. Results The DNA/RNA of the control group was 1.15±0.07.When the dose of γ-radiation was more than 1 Gy,DNA decreased obviously and the extent of the decrease of DNA was larger than that of RNA.The values of DNA/RNA of the groups that were irradiated with 1 Gy and 3 Gy were significantly different from those of the control group (P<0.01).The three-dimensional image manifested that after large dose of irradiation,the RNA in the cells gathered obviously while DNA were disrupted.When the dose was 8 Gy,the value of DNA/RNA was not different from that of the control group.When the dose was 8 Gy or higher,RNA was suppressed as seriously as DNA,and the value of DNA/RNA increased.There is certain relationship between the value of DNA/RNA and the dose of radiation. Conclusion After irradiated with different dose of γ-radiation,the ratio of DNA and RNA and the size of cells would change correspondingly. |
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