| LI Bing-yan,TONG Jian.Adverse effects of radon and its progeny on immune system in rats[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):200-202 |
| Adverse effects of radon and its progeny on immune system in rats |
| Received:October 16, 2003 |
| DOI： |
| KeyWords:Radon and its progeny Rat BALF Thymus and spleen Numbers and propertions of cells Spleen index DNA injury IL-6 mRNA |
| FundProject:江苏省重点实验室开放基金资助项目(KJS01009) |
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| Abstract:: |
| Objective To study adverse effects of inhaled radon and its progeny on immune system of rats. Methods Sprague-Dawley rats were exposed to radon and its progeny at cumulative doses up to 66,111 and 174 WLM (working level month),respectively.The numbers and types of cells in bronchoalveolar lavage fluids (BALF) were investigated.The spleen index was examined.The DNA migration in the thymus and spleen cells was detected with single cell gel electrophoresis(SCGE).RT-PCR was applied to determine the expression of IL-6 mRNA in BALF cells. Results It was shown that the proportion of lymphocyte of the exposed groups was significantly lower than that of the control group while the proportion of granulocyte was higher than that of the control group ( P< 0.05).The distance of DNA migration in the thymus and spleen cells increased in the 174WLM group.The spleen index of 111 WLM and 174 WLM groups decreased significantly compared with the control group.A high expression level of IL-6 mRNA in BALF cells was observed in the 174WLM group (P< 0.01)but there was no significant change in other two exposed groups compared with the control group. Conclusion The results suggest that in certain dose range radon and its progeny have adverse effect on structure and function of immune system of rats by affecting the proportion of lymphocyte and granulocyte in BALF, decreasing spleen index, and damaging the DNA of the thymus and spleen cells. |
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