| CAO Jian-ping,E. Fritz,ZHENG Si-ying.Effect of yeast TEL1 gene expression in A-T cells on chromosome aberration induced by radiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):192-195 |
| Effect of yeast TEL1 gene expression in A-T cells on chromosome aberration induced by radiation |
| Received:October 16, 2003 |
| DOI： |
| KeyWords:Ataxia-telangiectasia Yeast TEL1 gene Ionizing radiation Chromosome aberration |
| FundProject:国家自然科学基金资助项目(30170288); 教育部留学回国人员科研启动基金资助项目(教外司2002247) |
| Author Name | Affiliation | | CAO Jian-ping | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | E. Fritz | GSF-National Research Center for Environment and Health, Institute of Radiobiology, 87578 Neuherberg Gemany | | ZHENG Si-ying | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | 朱巍 | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | 罗加林 | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | 周献锋 | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | 王明明 | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China |
|
| Hits: 3096 |
| Download times: 1880 |
| Abstract:: |
| Objective To analyze the effect of yeast TEL1 gene expression in human A-T (ataxia-telangiectasia) cells on chromosome aberration induced by radiation. Methods Yeast TEL1 gene (TEL1rep),TEL1 fragment (deltaTELrep),pRep5 vector were stably transfected into A-T cells and pRep5 vector was stably transfected into GM639 cells.respectively.The stably transfected A-T and GM639 cells,namely,AT-TEL1,AT-deltaTEL1,AT-0rep,GM-rep,were grown selectively in Dulbecco's minimal essential medium (DMEM) containing 100 μgml hygromycin,while non-transfected A-T and GM639 cells were grown in DMEM containing 15%-20% FBS. 60Co γ-rays were used to irradiate the above cells at exponential phase.The irradiation dose was 0,1,3 Gy,respectively.A hundred metaphases were analyzed for microscopically detectable chromosome type and chromatid type aberrations. Results The chromosome numbers of all transfected,and non-transfected A-T,GM639 fobroblasts were aneuploid.Both spontaneous and radiation-induced chromosome aberration frequencies of A-T cells were higher than those of GM639 control cells.There is no significant difference in the spontaneous chromosome aberration between TEL1 gene stably transfected A-T cells (AT-TEL1) and non-transfected A-T cells (P>0.05).Chromosome aberration frequencies induced by radiation in A-TTEL1 cells were significantly lower than those of A-T cells (P<0.01).However,chromosome aberration frequencies induced by radiation in AT-deltaTEL1 and AT-rep cells were higher than those of A-T cells. Conclusion The expression of yeast TEL1 gene in human A-T cells can significantly decrease the chromosome aberration frequencies induced by radiation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
