| CHEN Jian-wei,HUANG Gang,LIU Jian-jun,et al.Effects of beta-rays on vascular endothelial cells[J].Chinese Journal of Radiological Medicine and Protection,2004,24(2):127-129 |
| Effects of beta-rays on vascular endothelial cells |
| Received:March 15, 2003 |
| DOI： |
| KeyWords:Beta-rays Endothelial cells Apoptosis Restenosis |
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| Abstract:: |
| Objective To elucidate the effects of beta-rays on vascular endothelial cells in the prophylactic treatment of vascular restenosis. Methods Cultured human umbilical vein endothelial cells (HUVEC) were irradiated with 2.5, 5.0, 10 and 20 Gy 32P beta-rays, separately. The proliferation of the cells was determined using MTT colorimetric assay. The ultrastructural features of HUVEC were observed by transmission electron microscopy. The cell cycle progression and apoptosis of HUVEC were analyzed by flow cytometry with a FACScan model. Results HUVEC proliferation was significantly inhibited in dose-dependent manner after 2.5, 5.0, 10, and 20 Gy beta-rays irradiation;the inhibitory rates were 5%(P <0. 01), 5%(P <0. 01), 7%(P <0. 01), and 11%(P <0. 01), respectively. Electron microscopy did not evidence typical apoptosis in HUVEC after 2. 5, 5. 0, 10 and 20 Gy beta-ray irraciation. No apoptotic peak corresponding to DNA degradation was noted at low or high beat-ray doses in the histogram. The apoptosis rate was less than 4. 4% in all irradiated groups and control group. But, HUVEC remained in G2/M phases after beta exposure. The percentages of G2/M phases were 17% (P <0. 01), 24%(P<0. 01), 29% ( P <0. 01), and 32%(P <0. 01), respectively, in the irradiated groups, compared with 13% in the control group. At the same time, G01< phases were arrested. The percentages of G0/G1 phases were 74%(P <0. 05), 70%(P <0. 01), 64%( P <0. 01), and 62%(P <0. 01), respectively, in the irradiated groups, compared with 76% in the control group. With a concomitant reduction in cell cycle progression into S phases, the percentages of S phases were 9% (P >0. 05), 6% (P<0. 01), 7% (P <0. 01), and 5% (P <0. 01), respectively in the irradiated groups, compared with 11% in the control group. Conclusion After 2. 5, 5. 0, 10, and 20 Gy beta-ray irradiation, HUVEC proliferation is significantly inhibited in dose-dependent manner, and cell cycle is arrested in G2/M, G0/G1, and S phases;however, no significant apoptosis appears in all experimental bata-ray doses. |
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