| YU Hong-sheng,FEI Cong-he,SHEN Fang-zhen,et al.Effects of low dose radiation combined with cyclophosphamide on tumor cell apoptosis, cell cycle and proliferation of bone marrow in tumor-bearing mice[J].Chinese Journal of Radiological Medicine and Protection,2004,24(2):117-119 |
| Effects of low dose radiation combined with cyclophosphamide on tumor cell apoptosis, cell cycle and proliferation of bone marrow in tumor-bearing mice |
| Received:November 10, 2003 |
| DOI： |
| KeyWords:Low dose radiation S180 sarcoma Apoptosis Cell cycle Bone marrow Proliferation |
| FundProject: |
| Author Name | Affiliation | | YU Hong-sheng | Department of Oncology, Qing Dao medical university Hospital, Qingdao 266003, China | | FEI Cong-he | Department of Oncology, Qing Dao medical university Hospital, Qingdao 266003, China | | SHEN Fang-zhen | Department of Oncology, Qing Dao medical university Hospital, Qingdao 266003, China | | 梁军 | Department of Oncology, Qing Dao medical university Hospital, Qingdao 266003, China |
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| Abstract:: |
| Objective To study the effect of low dose radiation (LDR) combined with cyclophosphamide on tumor cell apoptosis, cell cycle, and proliferation of bone marrow in mice tumor-bearing mice. Methods Kunming strain male mice were implanted with S180 sarcoma cells in the left hind leg subcutaneously as an experimental animal model. Five and 8 days after implantation, the mice were given 75 mGy whole-body γ-ray radiation and CTX(300 mg/kg) by intraberitoneal injection 36 hour after LDR. All mice were sacrificed to measure the tumor volume, tumor cell apoptoisis, and cell cycle;the proliferation of bone marrow was analyzed by flow cytometry. Results Tumor growth was significantly slowed down in the treated groups. The apoptosis of tumor cells increased significantly after LDR. The tumor cells were arrested in G1 phase in CTX and CTX+LDR groups, more significantly in the latter group than in the former group. Concentration of bone marrow cells and proliferation index in CTX+LDR group were higher than those in CTX group, although concentration of bone marrow cells in CTX and CTX+LDR groups were much lower than that in normal mice. Conclusion Low dose radiation combined with cyclophosphamide causes more significant G1-phase arrest than cyclophosphamide alone and enhances anti-tumor effect markedly. At the same time LDR significantly protects hematopoietic function of bone marrow, which is of practical significance as an adjuvant chemotherapy. |
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