| PAN Yao-zhen,SUN Cheng-yi,WANG Yu-zhi.Growth inhibition of human pancreatic cancer cells by lipofection mediated IGF-1R antisense oligodeoxynucletides in combination with ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(2):114-116 |
| Growth inhibition of human pancreatic cancer cells by lipofection mediated IGF-1R antisense oligodeoxynucletides in combination with ionizing radiation |
| Received:May 17, 2003 |
| DOI： |
| KeyWords:Ionizing radiation Lipofectin Apoptosis Antisense oligodeoxynucletides Gene transfection |
| FundProject:贵州省科学技术基金资助项目(黔基合计字20023106) |
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| Abstract:: |
| Objective To study the growth inhibition of human pancreatic cancer cells (PC-3) by lipofectin-mediated and ionizing radiation improving transfection of IGF-1R antisense oligodeoxynucletides(ASON) in vitro. Methods Colonigenicity of PC-3 cells in vitro after 60Co γ-radiation was observed for ascertaining their radiosensitivity and optimal radiation dose was selected according to the radiation sensitivity. PC-3 cells were transfected by two ways:①by lipofection-mediated IGF-1R ASON combined with ionizing radiation. ②by lipo-ASON alone without ionizing radiation. Cell growth was assessed by MTT method. The expression of IGF-1R at mRNA level was examined by RT-PCR. Flow cytometry was used to demonstrate apoptotic changes in lipo-ASON-treated cells. Results The inhibitory efficiency of lipo-ASON combined with ionizing radiation was higher than that without ionizing radiation (P< 0.05). The apoptotic efficiency and the decreased level of IGF-1R at mRNA were significantly improved ( P< 0.05). Conclusion Lipofectin-mediated and ionizing radiation-promoted transfection of IGF-1R antisense oligodeoxynucletides(ASON) significantly decreases IGF-1R at mRNA level and induces apoptosis of human pancreatic cancer cells in vitro. |
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