| FENG Wen-feng,HUANG Li-jin,QI Song-tao.Radiation-induced cerebral cell apoptosis in rats[J].Chinese Journal of Radiological Medicine and Protection,2004,24(2):111-113 |
| Radiation-induced cerebral cell apoptosis in rats |
| Received:April 15, 2003 |
| DOI： |
| KeyWords:X-irradiation Cerebral cells Apoptosis |
| FundProject: |
| Author Name | Affiliation | | FENG Wen-feng | Nanfang Hospital, The First Military Medical Universty, Guangzhou 510515, China | | HUANG Li-jin | Nanfang Hospital, The First Military Medical Universty, Guangzhou 510515, China | | QI Song-tao | Nanfang Hospital, The First Military Medical Universty, Guangzhou 510515, China | | 刘承勇 | Nanfang Hospital, The First Military Medical Universty, Guangzhou 510515, China | | 杨开军 | Nanfang Hospital, The First Military Medical Universty, Guangzhou 510515, China | | 任文德 | Nanfang Hospital, The First Military Medical Universty, Guangzhou 510515, China |
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| Abstract:: |
| Objective To study the influence of radiation on rat cerebral cells including neurons and gliocytes. Methods The rats were divided into control group and X-ray radiation groups with different doses. The apoptosis of cells at different time points after radiation was observed by optic microscopy, electron microscopy and DNA agarose gel electrophoresis. The double label method (in situ end-labeling of DNA strand breaks for labeling apoptotic cells, and immunhistochemistry for labeling cell type ) was used to label apoptotic neurons and gliocytes cells separately. Results The distinct morphological features of apoptosis and the DNA fragmentation ladders on agarose gel electrophoresis were seen in radiation groups. The rate of apoptosis in the adult rat brain was low. There were many apoptotic glial cells (about 93%) and a few apoptotic neurons (about 5%) after radiation. The apoptotic rate in high dose group was higher than that in low dose group. Conclusion Apoptosis can be induced by radiation in rat brain, the apoptotic rate increases with a increasing dose in the range of 2-8 Gy. The gliocytes are more sensitive to radiation-induced apoptosis than the neurons. |
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