| GUO De-huang,HAO Jing,LUO Qing-liang.Effect of rhSCF or rhSCF+rhG-CSF mobilized peripheral blood stem cells on acute radiation sickness in monkeys[J].Chinese Journal of Radiological Medicine and Protection,2004,24(2):108-110, 162 |
| Effect of rhSCF or rhSCF+rhG-CSF mobilized peripheral blood stem cells on acute radiation sickness in monkeys |
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| DOI： |
| KeyWords:rhSCF rhG-CSF Irradiation Peripheral blood stem cell transplantation |
| FundProject:国家“863”高技术基金资助课题(2001AA215401);全军“十五”指令基金资助课题(01L019) |
| Author Name | Affiliation | | GUO De-huang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | HAO Jing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | LUO Qing-liang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 熊国林 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 柳晓兰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 赵振虎 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 王欣茹 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 谢玲 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 陈松森 | 中国医学科学院基础医学研究所 | | 雒蓬轶 | 成都地奥九泓药厂 | | 王若竹 | 成都地奥九泓药厂 |
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| Abstract:: |
| Objective To observe the curative effect of rhSCF or rhSCF+rhG-SCF-mobilized peripheral blood stem cells(PBSC) on 7. 0 Gy γ ray-irradiated rhesus monkeys. Methods Fifteen healthy adult rhesus monkeys were divided into placebo control, rhSCF-mobilized, and rhSCF+rhG-CSF-mobilized groups. The two mobilized groups received rhSCF 50 μg·kg -1·d -1 for 3 days, then the rhSCF+rhG-CSF-mobilized group was further administered rhG-CSF 10 μg·kg -1·d -1for 5 successive days. The placebo control group was given 0. 9% sodium chloride at the same volume per kilogram. At day 0 of the irradiation, PBSCs were collected from all subjects. 3-4 hrs after irradiation, all animals received autologous PBSC infusion. Results rhSCF+rhG-CSF-mobilized PBSC accelerated the recovery of platelet and WBC counts, compared with rhSCF-mobilized PBSC, in which only higher platelet counts were observed. Bone marrow karyocyte culture demonstrated that only rhSCF+rhG-SCF-mobilized PBSC stimulated bone marrow karyocytes to form more CFU-GM, CFU-Mix, and CFU-MK. Histopathological study showed that there were more hematopoietic cells and less fat cells in the bone marrow of mobilized groups than in that of the placebo control group. Conclusion The infusion of rhSCF or rhSCF+rhG-CSF-mobilized PBSC improves the hematopoietic recovery in 7. 0 Gy γ ray irradiated rhesus monkeys. Two cytokine mobilization can get better effects. |
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