| SUN Xin-chen,WANG Jun-jie,ZHEN Yong-su.Enhancement of radiosensitivity by tetrandrine is associated with abrogation of cell cycle checkpoint[J].Chinese Journal of Radiological Medicine and Protection,2004,24(2):99-101 |
| Enhancement of radiosensitivity by tetrandrine is associated with abrogation of cell cycle checkpoint |
| Received:March 12, 2003 |
| DOI： |
| KeyWords:Tetradrine Radiosensitivity X-rays |
| FundProject:国家杰出青年基金资助项目(30025043);国家重大专项基金资助项目(2002AA2Z346D);国家“973”基金资助项目(2002CB513108) |
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| Abstract:: |
| Objective To investigate the radiosensitizing effect of tetrandrine (Tet) on human colon carcinoma HT-29 cells and its mechanism. Methods Clonogenic assay, flow cytometry and Western blotting were preformed in the experiments. In an animal model, tumor growth delay assay was used to determine the radiosensitivity. Results X-rays radiation markedly induced G2/M phase arrest in HT-29 cells. Tet abrogated the G2/M arrest induced by X-rays, and enhanced the cytotoxicity of X-irradiation by 1. 63-fold. Furthermore, X-rays increased expression of Chkl protein and decreased Cyclin B protein levels. Tet prevented increase of Chkl protein kinase and blocked decrease of Cyclin B1 protein. Mitotic index measurement showed that Tet dramatically stimulated X-irradiated cells to enter mitosis. The data showed that Tet enhanced suppression of the growth of colon carcinoma C26 by X-rays in BALB/c mice. Conclusion Tet is a potent abrogator of cell cycle checkpoint and enhances radiosensitivity in vitro and in vivo. |
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