| WU Xiao-min,YANG Ming-liang,HUANG Xiao-lan,et al.Effect of fucoidan on lymphocyte apoptosis and apoptosis-related gene expression induced by irradiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(1):33-34 |
| Effect of fucoidan on lymphocyte apoptosis and apoptosis-related gene expression induced by irradiation |
| Received:July 30, 2003 |
| DOI： |
| KeyWords:Fucoidan Irradiation Lymphocyte Bcl-2 Bax |
| FundProject:湖北省科技厅重点攻关资助项目(2001AA307B) |
| Author Name | Affiliation | E-mail | | WU Xiao-min | Departerment of Hygiene, School of Public Health, Wuhan University, Wuhan 430071, China | luoqiong@hbmu.edu.cn | | YANG Ming-liang | Departerment of Hygiene, School of Public Health, Wuhan University, Wuhan 430071, China | | | HUANG Xiao-lan | Departerment of Hygiene, School of Public Health, Wuhan University, Wuhan 430071, China | | | 阎俊 | Departerment of Hygiene, School of Public Health, Wuhan University, Wuhan 430071, China | | | 罗琼 | Departerment of Hygiene, School of Public Health, Wuhan University, Wuhan 430071, China | |
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| Abstract:: |
| Objective This study was conducted to explore the effects of fucoidan on rat splenic lymphocyte apoptosis and its Bcl-2 and Bax expression induced by 60Coγ-rays. Methods Rats were provided with different doses of fucoidan before whole-body irradiation with 9.0 Gy 60Coγ-rays.TUNEL and flow cytometry were used to investigate the effects of fucoidan on splenic lymphocyte apoptosis and immunohistochemical technique was used to detect the Bcl-2 and the Bax expression. Results Irradiation caused increase of apoptosis in rat splenic lymphocytes obviously,and the ratio of Bcl-2/Bax protein decreased significantly.Fucoidan could markedly inhibit the effects of radiation on apoptosis and the ratio of Bcl-2/Bax protein in a dose-dependent manner. Conclusion Fucoidan could inhibit rat splenic lymphocyte apoptosis induced by radiation,and its mechanism could be associated with its regulation on expressions of Bcl-2 and Bax proteins in splenic lymphocyte. |
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