| LIU Yao-wen,LUO Qing-liang,DONG Bo,et al.Effects of rhIL-11 on apoptosis of bone marrow cells in mice irradiated with 5.5 Gy 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2004,24(1):18-20 |
| Effects of rhIL-11 on apoptosis of bone marrow cells in mice irradiated with 5.5 Gy 60Co γ-rays |
| Received:March 07, 2003 |
| DOI： |
| KeyWords:Radiation injury rhIL-11 Hematopoietic cells Apoptosis |
| FundProject:全军“十五”指令性基金资助项目(01L019) |
| Author Name | Affiliation | | LIU Yao-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | LUO Qing-liang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | DONG Bo | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 郭德煌 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 熊国林 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 郝静 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 赵蓓蕾 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 葛平 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To evaluate the effect of rhIL-11 on apoptosis of bone marrow cells of mice irradiated with 5.5 Gy 60Coγ rays. Methods At 6,12,24 hours and on days 3,7 after irradiation,apoptosis of bone marrow cells was studied by means of in situ terminal labeling,flow cytometry and DNA electrophoresis.The expressions of P53 and Bcl-2 of bone marrow cells were evaluated by immunocytochemistry. Results (1)There was a significant descending trend of apoptotic ratio in rhIL-11-treated group 6 and 12 hours after TBI compared with the control group.(2)On day 3,P53 was expressed at a significantly lowere level ( P< 0.01),whereas Bcl-2 at a significantly higher level ( P< 0.01),in rhIL-11-treated groups than those of the placebo controls,respectively.On day 7,expression of Bcl-2 was still higher in rhIL-11-administered-before-TBI group ( P< 0.01). Conclusion rhIL-11 can promote recovery of murine BM cells by protecting them from apoptosis.The effects of rhIL-11 on apoptosis may have close relationship to the modulation of Bcl-2 and P53. |
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