| HU Ying-chun,XIANG Xiao-qiong,XU Qin-zhi,et al.Establishment of a metastatic model of human lung carcinoma induced by α particles in nude mice[J].Chinese Journal of Radiological Medicine and Protection,2004,24(1):16-18 |
| Establishment of a metastatic model of human lung carcinoma induced by α particles in nude mice |
| Received:April 24, 2003 |
| DOI： |
| KeyWords:Lung cancer Metastatic nude model |
| FundProject:国家重点基础研究专项资金资助项目(G1998051207) |
| Author Name | Affiliation | E-mail | | HU Ying-chun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | wudc@nic.bmi.ac.cn | | XIANG Xiao-qiong | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XU Qin-zhi | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 霍艳英 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 李邦印 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 段瑞峰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 李刚 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 张开泰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 吴德昌 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To establish a metastatic model of human lung carcinoma induced by α particles in nude mice. Methods A total of 9 BALB/C nude mice (5-6 weeks old) was inoculated subcutaneously (SC) with human tumor cell line BERP35T4.The tumor nodules developed from the nude mice were passaged for 3 times in nude mice via orthotopic implantation of histologically intact tissues. Results (1)Four of 9 nude mice developed (44.4%)neoplasm at the initial inoculation site.(2)one of four nude mice with neoplasm (25%) presented subcutaneous and lung metastasis.(3)Tumor tissue isolated from nude mice was characterized as squamous epithelial carcinoma with low differentiation.(4)CK3 and EMA staining were positive in cancer cells. Conclusion {A metastatic } model of human lung carcinoma induced by α particles in nude mice has been established via orthotopic implantation of histologically intact tissues. |
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