| LIU Qing-jie,CHEN De-qing,Julie RKorenberg.Radiation-induced translocation and dicentric chromosome aberrations in lymphocytes of umbilical cord blood analyzed by multicolor FISH method[J].Chinese Journal of Radiological Medicine and Protection,2004,24(1):13-15 |
| Radiation-induced translocation and dicentric chromosome aberrations in lymphocytes of umbilical cord blood analyzed by multicolor FISH method |
| Received:May 20, 2003 |
| DOI： |
| KeyWords:Multicolor FISH Translocation Dicentric Predicted value |
| FundProject:国家863计划项目(2003AA221081);卫生部科学研究基金资助项目(98246);美国能源部基金资助项目[DEFC0396ER62294、DEFG0301ER6326(JRK)] |
| Author Name | Affiliation | | LIU Qing-jie | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | CHEN De-qing | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | Julie RKorenberg | Cedars-Sinai Medical Center, UCLA, USA | | 陈晓宁 | Cedars-Sinai Medical Center, UCLA, USA | | 沈岩 | 国家人类基因组北方研究中心 |
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| Abstract:: |
| Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization (M-FISH) method. Methods The translocation and dicentric chromosome aberrations in lymphocytes of umbilical cord blood induced by different doses of 60Cogamma rays were analyzed by M-FISH.The specific telomeres and centromeres BAC clones of chromosomes 1,2,3,7,8,9,14 and 15 were used for M-FISH probes. Results (1)The dose-response curves of translocation and dicentric aberrations with absorbed dose levels accorded to linear-quadratic dose-response model.The translocation frequency was not equal to the dicentric frequency at most dose levels;(2)The proportion of cells with complete translocation but without unstable chromosome aberration decreased with increasing absorbed dose;(3)The observed values and the predicted values of chromosome aberrations,induced by 3 Gy gamma radiation,were not significantly different in most of the labeled chromosomes,but chromosome 9 had observed values significantly higher than predicted values ( P< 0.05 or P<0.01),and chromosome 15 vice versa ( P< 0.01). Conclusion The frequency of chromosome translocation induced by ionizing radiation is not equal to that of dicentric aberration.The frequencies of translocation and dicentric chromosome aberrations induced by ionizing radiation are at random for most chromosomes. |
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