| HUANG Yue-cheng,CAI Jian-ming,HAN Ling.Expression of MDM2 in an acute lymphocytic leukemia mice model induced by γ-radiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(1):6-9 |
| Expression of MDM2 in an acute lymphocytic leukemia mice model induced by γ-radiation |
| Received:June 27, 2003 |
| DOI： |
| KeyWords:MDM2 Leukemia Western blot In situ hybridization |
| FundProject:国家自然科学基金资助项目(30170282) |
| Author Name | Affiliation | | HUANG Yue-cheng | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | CAI Jian-ming | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | HAN Ling | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | 高福 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | 崔建国 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | 高建国 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China |
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| Abstract:: |
| Objective To investigate the role of the MDM2 in the process of carcinogenesis induced by γ-rays and its molecular mechanisms. Methods Animal model of radiation-induced leukemia was established by γ-irradiation.According to the histological and morphological results,mice tissues were divided into three groups:cancerization group,incancerization group and control group.Expression of MDM2 protein and mRNA in thymus/bone marrow was detected with Western blot and in situ hybridization (ISH),respectively.We also examined the protein phosphorylation level of MDM2 protein by immunoprecipitation (IP).PCR-SSCP was performed to detect gene mutation. Results A mice leukemia model was successfully established as verified by pathological findings and confirmed by transplantation test in nude mice.The protein expression in thymus/bone marrow in irradiation groups was significantly higher than that in controls ( P<0.05).But the expression level was not statistically different between the cancerization group and the incancerization group.The MDM2 was found to be hyperphosphorylated in the cancerization group as compared with other groups.No gene mutation was detected by SSCP/silver-staining assay in the tumor samples. Conclusion MDM2 may be involved in the development and progression of leukemia induced by γ-irradiation.The over-expression but not gene mutation may be responsible for malignant transformation induced by radiation.Phosphorylation is at least partly attributed to activation of MDM2. |
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