| SHI Chunmeng,CHENG Tianmin.Effects of dermis-derived multipotent stem cells on the recovery of hematopoiesis in irradiated rats[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):435-438 |
| Effects of dermis-derived multipotent stem cells on the recovery of hematopoiesis in irradiated rats |
| Received:October 27, 2002 |
| DOI： |
| KeyWords:Multipotent stem cells Hematopoiesis Radiation in jury Dermis Rats |
| FundProject:国家重点基础研究发展规划“973”资助项目(G1999054205) |
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| Abstract:: |
| Objective To study the effects of dermis-derived mul tipotent stem cells on the recovery of hematopoiesis in irradiated rats. Methods The multipotent stem cells were isolated from dermis of new-born rats and were confirmed by phenotypic analysis and multilineage differ entiation.The confluent multipotent stem cell layer was used to observe the effe cts on the growth of bone marrow granulocyte/macrophage progenitors (CFU-GM) an d erythroid progenitors(CFU-E) in vitro;Then dermis-derived multipotent st em cells were infused into-tailveins of 5 Gy-irradiated Wistar rats.The survi val rate,numbers of CFU-GM,CFU-E and nucleated cells in bone marrow and WBCs,R BCs in peripheral blood of the rats were selected as parameters to evaluate the hematopoiesis recovery. Results The dermis-derived multipotent s tem cell layer could support the growth of CFU-GM and CFU-E hematopoietic colo nies in vitro. Transplantation of dermis-derived multipotent cells increase d the survival rate and the numbers of nucleated cells,CFU-GM,CFU-E coloni es in bone marrow and white blood cells in peripheral blood in recipients versus rats of the irradiation group without such treatment. Conclusion The dermis-derived multipotent stem cells can promote hematopoiesis recovery in irradiated rats and may provide a new source for stem cell therapy. |
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