| HE Dongchu,WANG Xiaohong,HE Hui,et al.Effect of Digan oral liquid on expression of cell cycle progression and apopto sis-related genes in mice with radiation lesions[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):432-434 |
| Effect of Digan oral liquid on expression of cell cycle progression and apopto sis-related genes in mice with radiation lesions |
| Received:January 04, 2003 |
| DOI： |
| KeyWords:Digan oral liquid Radiation injury Apoptosis Gene expression |
| FundProject:湖北省自然科学基金资助项目(97J080) |
|
| Hits: 3422 |
| Download times: 1975 |
| Abstract:: |
| Objective To study the effect of Digan oral liquid (DGOL) on expression of cell cycle prog ression(cyclinD1)- and apoptosis(bcl-2 and bax)- related genes in bone marr ow of mice with radiation lesions. Methods After the radiation lesion model(given 6 0 Gy X-rays on ce by a linear accelerator) was established,the animals were fed with 0 2 ml/10 g of either small dose (100%) or large dose (200%) DGOL twice a day for 8 days. On the ninth day cyclinD1,bcl-2 and bax mRNA expression levels in marrow MNC of model mice were measured by in situ hybridization. Results In the DGOL group,the bax mRNA expression was significantly decreased (P<0.01),but cyclinD1 and bcl-2 mRNA were significantly increased (P<0.01) t han those in the control group. Conclusion DGOL can restrain radiation lesion due to its enhancing effects on cyclinD1 an d bcl-2 mRNA expression,and inhibitory effect on bax mRNA expression.In this wa y,it prevents the blood cells from injury,promotes immunity,and protects the org anism.This result provides a theoretical basis for clinical application of Diga noral liquid. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
