| TIAN Mei,JIN Guanghui,PIAO Chunji.Study on construction of pEgr-hPTEN expression vector induced by irradiation and its anti-tumor effect in vitro[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):423-426 |
| Study on construction of pEgr-hPTEN expression vector induced by irradiation and its anti-tumor effect in vitro |
| Received:June 27, 2003 |
| DOI： |
| KeyWords:hPTEN pEgr-hPTEN expression vector Gene -radio therapy |
| FundProject:国家自然科学基金资助项目(30170290) |
| Author Name | Affiliation | | TIAN Mei | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | JIN Guanghui | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | PIAO Chunji | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | 刘林林 | 吉林大学第二医 | | 李修义 | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To clone the cDNA of human tumor suppressor gene-PTEN ,construct pEgr-hPTEN e xpression vector induced by irradiation and study its inhibitory effect on proli feration of malignant glioma cell line SHG-44 transfected steadily with pEgr-h PTEN after different doses of X-ray irradiation. Methods A DNA fragment about 1 200 bp ,PTEN, was amplified from human placenta tissues by using RT-nes ted PCR and was cloned into pUCm-T vector after automatic sequencing, the n the fragment was inserted into a vector pcDNA3 1-Egr to construct an express i on vector pEgr-hPTEN. pEgr-hPTEN was transfected into SHG-44 cells in vitro . Stably transfected cell line SHG-44-sPTEN was selected through G418. The inhi bitor effect on SHG-44-sPTEN was observed after different doses of X-ray irra diation in vitro. Results The PTEN cDNA has been cloned correctly and its expre ssion vector pEgr-hPTEN was also constructed. Growth of SHG-44 cells was inhi bited significantly by stable pEgr-hPTEN transfection combined with X-ray irra diation. With the increase of dose , the inhibitory effect was enhanced within 5 Gy. Conclusion Human tumor suppressor gene-PTEN cDNA has been cloned and its e xpression vector has been constructed. The tumor was inhibited significantly by gene-radiotherapy in vitro. The result provides the theoretical and experim ental basis for improvement of clinical radiotherapeutic effect on tumors . |
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