| JIN Shunzi,HE Shujie,LIU Shuzheng.Experimental studies on the activation of NF-κB and its signal pathway induced by low dose radiation[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):408-410 |
| Experimental studies on the activation of NF-κB and its signal pathway induced by low dose radiation |
| Received:March 03, 2003 |
| DOI： |
| KeyWords:Low dose radiation Signal pathway NF-κB I κB Calphostin C |
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| Abstract:: |
| Objective To observe the effect of low dose radiation (LDR) on the activation of NF-κB and the possible mechanism of its signal tra nsduction. Methods Electrophoretic mobility shift assay (EMSA) and immunohistochemistry were used to detect the changes of DNA-binding activity o f NF-κB and the nuclear translocation of p65 in EL-4 cells,respectively.Meanw hile,LipofectAMINE-mediated transient transfection was used to carry vacant pla smid and NF-κB-luciferase-containing plasmid into NIH3T3 cells,with or witho ut the addition of PKC-specific blocking agent Calphostin C,to detect the expre ssion of luciferase after irradiation. Results X-rays of 0.075 Gy increased the activities of p50/p65 a nd p50/p50 dimers,especially the former.The translocation of p65 into nuclei inc reased and IκBα exhibited the opposite changes.Calphostin C reversed the effec t of 0.075 Gy radiation on the activity of NF-κB. Conclusion The activation of NF-κB induced by LDR might be effec ted via the PKC pathway,resulting in the facilitation of the expression of cytok ines and up-regulation of immune functions,which might be involved in the mecha nisms of excitatory effects of LDR. |
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