| LIU Guangwei,DONG Lihua,LIU Yang,et al.Adaptive response of spermatogenic cell apoptosis selectively induced by low dos e X-ray irradiation in mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):405-408 |
| Adaptive response of spermatogenic cell apoptosis selectively induced by low dos e X-ray irradiation in mice |
| Received:March 03, 2003 |
| DOI： |
| KeyWords:Low dose radiation Adaptive response Apoptosis Spermatogenic cells |
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| Author Name | Affiliation | | LIU Guangwei | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | DONG Lihua | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | LIU Yang | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 吕喆 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 刘淑春 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 龚守良 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective The adaptive response of spermatogenic cell apoptosis induced by whole-body X- r ay irradiation at low doses was studied in mice. Methods Kunming m ale mi ce were irradiated with an inductive dose (D1:75 mGy) and/or a challenging dose (D2:1 0,2.0 or 3.0Gy).Different kinds of spermatogenic cells were separated using d ensity gradient centrifugation and their apoptotic percentages were analysed usi ng flow cytomentry (FCM). Results When the mice were irradiated w ith D1 6 h before irradiation with D2,the apoptotic percentages of the spermatog onia and spermatocytes declined rapidly as compared with those in the groups irr adiated with D2 only,and those of spermatids and spermatozoa showed no significa nt ch anges.When the interval times between D1 and D2 was 3,6,12 or 24 h,the apoptotic percentages in spermatogonia and spermatocytes reduced early,significantly and continued for a longer duration after smaller D2(1.0 and 2.0 Gy) irradiation,w hile the apoptotic percentages did not change after larger D2(3.0 Gy) irradiati on. Conclusion The adaptive response of apoptosis in spermatogonia and spermatocytes could be selectively induced by low dose X-ray irradiation.Th e adaptive response could be closely related to the D2 dose and interval time be tween D1 and D2. |
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