| LIU Xiaodong,MA Shumei,Lü Zhe,et al.Effects of low dose radiation on secretion of IL-12 a nd expressions of CD2,CD48 in mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):402-405 |
| Effects of low dose radiation on secretion of IL-12 a nd expressions of CD2,CD48 in mice |
| Received:March 03, 2003 |
| DOI： |
| KeyWords:LDR IL-12 CD2 CD48 |
| FundProject:国家自然科学基金资助项目(39570188) |
| Author Name | Affiliation | | LIU Xiaodong | MH Radiobiology Research Unit, Norman Be thune Medical Faculty, Jilin University, Changchun 130021, China | | MA Shumei | MH Radiobiology Research Unit, Norman Be thune Medical Faculty, Jilin University, Changchun 130021, China | | Lü Zhe | MH Radiobiology Research Unit, Norman Be thune Medical Faculty, Jilin University, Changchun 130021, China | | 刘淑春 | MH Radiobiology Research Unit, Norman Be thune Medical Faculty, Jilin University, Changchun 130021, China | | 刘树铮 | MH Radiobiology Research Unit, Norman Be thune Medical Faculty, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To observe the changes in the secretion of IL-12 and the expressions of CD2 and CD48 in mice after low dose irradiation an d to analyses the possible relationship between them. Methods North ern blot and ELISA were used to detect the mRNA and the protein level of IL-12 respectively.Flow cytometry(FCM) was adopted to detect the changes in CD2 and CD 48 expression. Results (1)Changes in IL-12 mRNA levels of both IL-12 p35 and p40 subunits increased and reached their peaks at 1 h(131% and 19 2% of sham-irrad respectively) after WBI with 0.075 Gy X-rays and IL-12 secr etion by macrophages was found to increase from 4 h to 8 h after exposure (P<0.05).(2)CD2,CD48 expression:FCM data showed CD2 expression in thymocytes i ncreased beginning from 4 h,reaching its peaks, at 8 h after irradiation,while the increase of CD48 expression in peritoneal macrophages was found from 2 h and maintained at high level up to 48 h (P<0.05～0.01).The results of dose -effect study at 4 h after irradiation illustrated up-regulated expression of CD2 and CD48 after 0.050,0.075,0.100,0.200 Gy irradiation (P<0.05). Conclusion LDR might up-regulate the expression of IL-12,and the consistence of CD2 and CD48 with IL-12 changes suggested that they might part icipate in the immunological regulation by increased secretion of IL-12. |
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