| JU Guizhi,WANG Xiaomei,FU Shibo.Effect of ionizing radiation on protein expressions of p16,Cyclin D1 and CDK4 in thymocytes and splenocytes of mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(6):399-401 |
| Effect of ionizing radiation on protein expressions of p16,Cyclin D1 and CDK4 in thymocytes and splenocytes of mice |
| Received:March 03, 2003 |
| DOI： |
| KeyWords:Ionizing radiation p16 Cyclin D CDK4 |
| FundProject:国家自然科学基金资助项目(30270346) |
| Author Name | Affiliation | | JU Guizhi | MH Radiobiology Research Unit, Medical Cent er in Memorial of Norman Bethune, Jilin University, Changchun 130021, China | | WANG Xiaomei | MH Radiobiology Research Unit, Medical Cent er in Memorial of Norman Bethune, Jilin University, Changchun 130021, China | | FU Shibo | MH Radiobiology Research Unit, Medical Cent er in Memorial of Norman Bethune, Jilin University, Changchun 130021, China | | 刘树铮 | MH Radiobiology Research Unit, Medical Cent er in Memorial of Norman Bethune, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To investigate the effect of ionizing radia tion on the expressions of p16,CyclinD1 and CDK4 in thymocytes and splenocytes o f mice. Methods Fluorescent staining and flow cytometric analysis were employed for measurement of protein expressions. Results It was found in time course experiments that the expression of p16 protein incre ased significantly at 8,24 and 48 h in thymocytes (P<0.05,P<0.01 and P<0.05, respectively) and at 24 h in splenocytes (P<0.05) after whol e-body irradiation (WBI) with 2.0 Gy X-rays.However,the expression of CDK4 pr otein decreased significantly from 8 to 24 h in thymocytes (P<0.05-P<0.01) and from 8 to 72 h in splenocytes (P<0.05-P<0.01).In dose-effe ct experiments,it was found that the expression of p16 protein in thymocytes and splenocytes increased significantly at 24 h after WBI with the doses of 1.0,2.0 and 4.0 Gy(P<0.05-P<0.01),whereas the expression of CDK4 protein decreased significantly with the dose of 2.0 Gy for thymocytes (P<0.05) a nd with the doses from 0.5 to 6.0 Gy for splenocytes (P<0.05-P<0.01 ).The expression of CyclinD1 protein decreased markedly in both thymocytes and s plenocytes after exposure. Conclusion The results indicate that th e expression of p16 protein in thymocytes and splenocytes could be induced by io nizing radiation,and the p16-CyclinD1/CDK4 pathway might play an important role for G1 arrest of thymocytes induced by X-rays. |
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