| PAN Qi,HE Dongchu,WU Jiangping.Effect of Digan oral liquid on apoptosis and expression of mRNA of some apoptosis-related genes in the spleen tissue of mice with radiation injury[J].Chinese Journal of Radiological Medicine and Protection,2003,23(5):341-342 |
| Effect of Digan oral liquid on apoptosis and expression of mRNA of some apoptosis-related genes in the spleen tissue of mice with radiation injury |
| Received:March 18, 2003 |
| DOI： |
| KeyWords:Digan oral liquid (DGOL) Radiation injury Apoptosis Gene expression |
| FundProject:湖北省自然科学基金资助项目(97J080) |
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| Abstract:: |
| Objective To study the effect of Digan oral liquid(DGOL) on apoptosis and expression of mRNA of some apoptosis-related genes in the spleen tissue of mice with radiation injury.Methods After irradiation with 7.5 Gy X- rays by a linear accelerator,the animals were fed with or without 0.2 ml/10 g of 100% DGOL.Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) in situ hybridiation.Results The apoptotic ratio and bax-2 mRNA expression were significantly decreased (P<0.01) in spleen tissue,whereas bcl-2 mRNA expression was significantly increased (P<0.01) in spleen tissue of the DGOL group than those in the control group. Conclusion DGOL can restrain the apoptosis in spleen tissue of mice with radiation injury, promote proliferation of hemopoietic cells, and elevate immunological function of the organism via enlancing bcl-2 expression and decreasing bax-2 mRNA expression. These results provide theoretical basis for clinical application of DGOL. |
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