| WANG Liansheng,HUANG Jun,MA Genshan.Inhibitory effect of gamma radiation on cultured rabbit vascular smooth muscle cells[J].Chinese Journal of Radiological Medicine and Protection,2003,23(5):337-340 |
| Inhibitory effect of gamma radiation on cultured rabbit vascular smooth muscle cells |
| Received:September 23, 2002 |
| DOI： |
| KeyWords:Smooth muscle cells Ionizing radiation Proliferation Apoptosis Micronucleus |
| FundProject:江苏省科委基金资助项目(BK9901411);南京医科大学科技基金资助项目(MA9906) |
| Author Name | Affiliation | | WANG Liansheng | Department of Cardiovascular Medicine, The 1 st Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China | | HUANG Jun | Department of Cardiovascular Medicine, The 1 st Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China | | MA Genshan | Department of Cardiovascular Medicine, The 1 st Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China | | 杨志健 | Department of Cardiovascular Medicine, The 1 st Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China | | 周振英 | 江苏省肿瘤医院流式细胞检测中心 | | 姚玉宇 | 南京医科大学病理学教研室 |
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| Abstract:: |
| Objective Using in vitro cultured smooth muscle cells (SMCs) to examine the gamma radiation dose-response relationship and to investigate the cellular mechanisms by which radiation inhibits proliferation of SMCs. Methods Quiescent SMCs from rabbit illiac artery were irradiated with 2.5-30 Gy gamma rays,and immediately cultured in DMEM medium with 10% FCS for 4,24 and 72 hrs respectively. 3H-TdR,flow cytometry,micronucleus test,electron microscopy were carried out to determine the impacts of gamma radiation on DNA synthesis,cell cycle phase,and cellular damage of SMCs. Results Gamma radiation significantly inhibited SMCs proliferation in a dose-dependent manner and caused cell cycle G0/G1 arrest at the doses above 2.5 Gy.A low dose (<2.5-5 Gy) could increase SMCs apoptosis at 24 hrs after irradiation,while a high dose could cause their death. Conclusions Ionizing radiation probably inhibits SMCs proliferation dose-dependently and causes cell cycle G0/G1 arrest.At low dose level gamma radiation can induce apoptosis,while at high dose level it can kill SMCs directly. |
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