| PAN Xiaoyan,HE Jiliang,ZHANG Meibian.Study of synergistic DNA-damaging effects of low-intensity 2450 MHz microwave and 4-nitroquinoline on human lymphocytes in vitro[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):301-303 |
| Study of synergistic DNA-damaging effects of low-intensity 2450 MHz microwave and 4-nitroquinoline on human lymphocytes in vitro |
| Received:October 21, 2002 |
| DOI： |
| KeyWords:Microwave 4-NQO Comet assay Synergistic effect Human lymphocytes |
| FundProject:浙江省自然科学基金资助项目(300434);浙江省科技厅国际合作项目(012104) |
| Author Name | Affiliation | E-mail | | PAN Xiaoyan | Institute of Environmental and Occupational Health, Medical School of Zhejiang University, Hangzhou 310006, China | he-jiliang@hotmail.com | | HE Jiliang | Institute of Environmental and Occupational Health, Medical School of Zhejiang University, Hangzhou 310006, China | | | ZHANG Meibian | Institute of Environmental and Occupational Health, Medical School of Zhejiang University, Hangzhou 310006, China | | | 金力奋 | Institute of Environmental and Occupational Health, Medical School of Zhejiang University, Hangzhou 310006, China | |
|
| Hits: 2578 |
| Download times: 1707 |
| Abstract:: |
| Objective To study the synergistic DNA-damaging effects of low-intensity 2 450 MHz microwave(MW, 5.0 mW/cm2)and 4-nitroquinoline(4-NQO)on human lymphocytes. Methods The DNA damages of human lymphocytes treated with microwave and 4-NQO were detected using the comet assay.Three combination-exposure ways of MW and 4-NQO were used:MW irradiation before 4-NQO exposure(MW+4-NQO), simultaneous exposure to MW and 4-NQO(MW-4-NQO), and MW irradiation after 4-NQO exposure(4-NQO+MW). Results The differences of DNA-damage indexes (comet tail length, comet shape and total comet length)between MW group and control group were not significant (PP>0.05).When the dose of 4-NQO was 50 μmol/L or25 μmol/L, the differences of DNA-damage indexes between MW+4-NQO group and corresponding 4-NQO group were significant(PP<0.05 or PP<0.01).The differences of DNA-damage indexes between MW-4-NQO group or 4-NQO+MW group and corresponding 4-NQO group were not significant(PP>0.05). Conclusion Low-intensity 2 450 MHz microwave does not induce DNA-damage directly, but could enhance the DNA-damaging effects induced by 4-NQO when human lymphocytes have been treated with MW irradiation before 4-NQO exposure. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
