| WEI Qichun,ZHENG Shu.Establishment and cell cycle distribution pattern of a radioresistant subline from human lung cancer D6 cell line[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):267-269 |
| Establishment and cell cycle distribution pattern of a radioresistant subline from human lung cancer D6 cell line |
| Received:January 22, 2003 |
| DOI： |
| KeyWords:Lung Neoplasm Radiation Radiosensitivity Cell cycle |
| FundProject:浙江省医药卫生科研基金资助项目(2000B117);浙江省中医药科研基金资助项目(2002Y002) |
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| Abstract:: |
| Objective To establish a radioresistant cell subline from a human D6 lung cancer cell line and investigate the mechanism of radioresistance. Methods D6 human NSCLC cells were exposed to X-rays generated by a linear accelerator(650 cGy per fraction).After a total exposure dose of 5 200 cGy, a monoclone was obtained.The radiosensitivity and cell cycle distribution of this clone, together with its parent D6 cells, were measured by clonogenic assay and flow cytometry. Results The new clone, namely D6-R subline, had a higher D0(D0=2.08 Gy) and a broader initial shoulder(Dq=1.64 Gy, N=2.20)than those of the parent D6 cell line (D0= 1.84 Gy、Dq=0.34 Gy, N=1.20), being 1.65-fold increase in radioresistance as regards to the SF 2.The D6-R subline also showed higher percentage of cells in S phase(53.4% vs 37.8%), but lower percentages in G1(44.1% vs 57.2%) and G2/M(2.5% vs 5%)phases. Conclusion The new subline D6-R is more radioresistant as compare to its parent D6 cell line, and has a different cell cycle distribution. |
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