| FAN Yan,WU Shiliang,XU Lan.Effect of taurine on expressions of MMP-2 in K562 leukaemia cell line exposed to γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):253-255 |
| Effect of taurine on expressions of MMP-2 in K562 leukaemia cell line exposed to γ-rays |
| Received:December 16, 2002 |
| DOI： |
| KeyWords:γ-rays Taurine K562 Leukaemia Matrix Metalloproteinase(MMP-2) |
| FundProject:国防科工委国防预研基金资助项目(Y6920004);江苏省高校自然科学基金资助项目(01KJB310001) |
| Author Name | Affiliation | E-mail | | FAN Yan | Suzhou University, Department of Biochemistry and Molecular Biology, Suzhou 215007, China | Shiliangwu@hotmail.com | | WU Shiliang | Suzhou University, Department of Biochemistry and Molecular Biology, Suzhou 215007, China | | | XU Lan | Suzhou University, Department of Biochemistry and Molecular Biology, Suzhou 215007, China | | | 仇灏 | Suzhou University, Department of Biochemistry and Molecular Biology, Suzhou 215007, China | | | 周迎会 | Suzhou University, Department of Biochemistry and Molecular Biology, Suzhou 215007, China | |
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| Abstract:: |
| Objective To study the effect of γ-irradiation on expressions of MMP-2 in leukaemia cells and the suppressive effect of taurine(Tau) on irradiated tumour cells in terms of cellular level. Methods The cells in the control group and Tau (50 mg/L, 100 mg/L, 200 mg/L)groups were irradiated with 15 Gy γ-rays. The expressions of MMP-2 were examined through Western-blotting after handled with gel-loading buffer within 12 h. Results The expressions of MMP-2 were enhanced evidently in the positive control group, while they were less in the negative control group. In the Tau(50 mg/L, 100 mg/L, 200 mg/L)groups, the expressions of MMP-2 were diminished in turns, and they were almost identical between the negative control group and the Tau 200 mg/L group. Conclusion Irradiation with γ-rays at a dose of 15 Gy can significantly stimulate the expressions of MMP-2 in K562 cells; Tau can inhibit the expressions of MMP-2 and its effect depends on to its dosage; Tau can inhabit the invasiveness and migration of irradiated tumour cells, so it has the biologic protective and therapeutic effects. |
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