| GU Qingyang,WANG Dewen,PENG Ruiyun.MAPK signal transduction pathway may be involved in the inhibitory effect of bFGF on radiation-induced apoptosis in HUVEC[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):244-246 |
| MAPK signal transduction pathway may be involved in the inhibitory effect of bFGF on radiation-induced apoptosis in HUVEC |
| Received:January 06, 2003 |
| DOI： |
| KeyWords:bFGF Endothelial cells Apoptosis MAPK |
| FundProject:全军“九五”医药卫生科研基金资助项目(96Z008) |
| Author Name | Affiliation | | GU Qingyang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | WANG Dewen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | PENG Ruiyun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 董波 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 王兆海 | 解放军三○七医院 | | 刘杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 邓桦 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 王水明 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 姜涛 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To study the effect of bFGF on radiation-induced apoptosis in vascular endothelial cells and its signal transduction pathways. Methods Primary cultured of HUVEC (human umbilical vein endothelial cells) was irradiated with 60Coγ-rays to establish a cell apoptosis model. Flow cytometry with Annexin-V- FITC+ PI labeling was used to evaluate cell apoptosis. Different amounts of bFGF and MEK inhibitor U0126 were used to study their effects on radiation-induced endothelial cell apoptosis. Results We found that bFGF could inhibit radiation-induced apoptosis in HUVEC, i.e., the apoptotic rate decreased from (35.22±5.5)% to (11.67±3.8)%.Meanwhile, U0126 could partly block the anti-apoptosis effect of bFGF, the apoptotic rate increased from (10.59±1.6)% to (17.96±1.4)%. Conclusion MAPK signal transduction pathway may be involved in the inhibitory effect of bFGF on radiation-induced apoptosis in HUVEC. |
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