| YANG Wei,WU Congmei,LI Xiuyi.Experimental study on radiation-inducible expression and anti-tumor effect of pEgr-IFNγ recombinant plasmid[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):238-240 |
| Experimental study on radiation-inducible expression and anti-tumor effect of pEgr-IFNγ recombinant plasmid |
| Received:February 07, 2003 |
| DOI： |
| KeyWords:pEgr-IFNγ recombinant plasmid X-ray B16 melanoma Gene-radiotherapy |
| FundProject:国家自然科学基金资助项目(39970229) |
| Author Name | Affiliation | | YANG Wei | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | WU Congmei | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | LI Xiuyi | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 李秀娟 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 田梅 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 刘林林 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 朴春姬 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | 龚守良 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To study the radiation-inducible expression and the anti-tumor effect of pEgr-IFNγ recombinant plasmid in mice bearing melanoma. Methods The pEgr-IFNγ plasmid was injected locally into the tumor in the mice, and the tumors were irradiated with X-rays 36 hours later. The tumor growth rate at different times and mean survival period of the mice were observed. The IFNγ mRNA level in the tumor was detected with RT-PCR, 3 days after irradiation, and the concentration of IFNγ in the serum of the mice was detected by ELISA 1, 3 and 5 days after irradiation. Results The IFNγ mRNA level in the tumor of mice in the gene-radiotherapy group was significantly higher than that in the reconmbinant plasmid group 3 days after irradiation. The IFNγ concentration in the serum of mice in the gene-radiotherapy group was higher than that in the recombinant plasmid group and the control group 1 and 3 days after irradiation. The tumor growth rate in the group of plasmid injection followed by 5 Gy irradiation for four times was significantly lower than that in the group of plasmid injection followed by 20 Gy irradiation 9-15 days after irradiation, and the mean survival period was also longer. Conclusions The anti-tumor effect of plasmid injection followed by lower dose irradiation for several times is better than that by higher dose irradiation. By inducing higher expression of IFNγ gene in the tumor, pEgr- IFNγ gene-radiotherapy could increase the concentration of IFNγ in the serum, and therefore the body's immunologic function and anti-tumor ability are enhanced. |
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