| ZHANG Bo,SU Yongping,LIU Xiaohong.Differential proteomic analysis of mouse intestinal epithelium irradiated by γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):234-237 |
| Differential proteomic analysis of mouse intestinal epithelium irradiated by γ-rays |
| Received:January 30, 2003 |
| DOI： |
| KeyWords:Ionizing radiation Intestinal epithelial cells 2-D electrophoresis Peptide mass fingerprinting |
| FundProject:国家自然科学基金资助项目(30230360) |
| Author Name | Affiliation | | ZHANG Bo | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | SU Yongping | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | LIU Xiaohong | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 艾国平 | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 冉新泽 | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 魏永江 | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 王军平 | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 程天民 | Institute of Combine Injury of PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
|
| Hits: 2823 |
| Download times: 2108 |
| Abstract:: |
| Objective For elucidating the molecular mechanism of reconstruction of intestinal epithelium damaged by ionizing radiation, the proteomes of murine intestinal epithelium from normal and irradiated mice were compared by 2-D electrophoresis. Methods Histopathologic sections of whole small intestine made from BALB/c mice 3 h and 72 h after total-body irradiation were stained with hematoxylin-eosin.Intestinal epithelial cells were isolated from normal and irradiated mice.The total protein samples prepared by one-step method were used in 2-D electrophoresis, the protein maps were compared and the differential spots were detected with PDQuest analysis software.Twenty-eight different spots were cut off from the gels, digested in gel with trypsin, measured with MALDI-TOF-MS and searched in databased. Results Small intestinal epithelium was damaged as early as 3 h after irradiation, and reconstructed 72 h later.After Coomassie-staining, the 2-DE image analysis by PDQuest software detected 638±39 protein spots in normal mice group, 566±32 spots in 3 hours post irradiation group, and 591±29 spots in 3 days post irradiation group.The 2-DE images showed that proteomes of intestinal epithelium were altered with γ-irradiation.The proteins identified by peptide mass fingerprinting involved in cellular events, including signal transduction, metabolism and oxidative stress responses. Conclusions Gamma-irradiation can induce the protein expression of intestinal epithelium.The technique of 2-D electrophoresis is a useful tool in the study of molecular mechanism of radiation damage. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
