| FAN Baoxing,ZHANG Kaitai,SUN Jingfen.Identification of differentially expressed genes in different malignant transformation phases of BEP2D cell line[J].Chinese Journal of Radiological Medicine and Protection,2003,23(4):231-233 |
| Identification of differentially expressed genes in different malignant transformation phases of BEP2D cell line |
| Received:January 06, 2003 |
| DOI： |
| KeyWords:Alpha-particles SSH cDNA microarray BEP2D cell |
| FundProject:国家重点基础研究发展规划973基金资助项目(G1998051208) |
| Author Name | Affiliation | E-mail | | FAN Baoxing | Department of Respiratory Disease, 301 Hospital of PLA, Beijing 100853, China | fanbaox@sohu.com | | ZHANG Kaitai | 100850, 北京放射医学研究所 | | | SUN Jingfen | 100850, 北京放射医学研究所 | | | 项小琼 | 100850, 北京放射医学研究所 | | | 谢玲 | 100850, 北京放射医学研究所 | | | 胡迎春 | 100850, 北京放射医学研究所 | | | 王升启 | 100850, 北京放射医学研究所 | | | 吴德昌 | 100850, 北京放射医学研究所 | |
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| Abstract:: |
| Objective To screen and identify differentially expressed genes in different malignant transformation phased of BEP2D cell line induced by alpha-particles. Methods Suppression subtractive hybridization(SSH) and cDNA microarray were performed. Results Three suppression subtractive cDNA libraries were constructed.BEP2D cDNA library contained 416 clones, R15H20 cDNA library contained 301 clones, and R15H35 cDNA library contained 586 clones.After confirmed by cDNA microarray, the positive rates in BEP2D cDNA microarray were 90.4%, 21.6% and 19.7%;in R15H20 cDNA microarray, 8.6%, 93.8% and 31.6%;and in R15H35 cDNA microarray, 23.5%, 18.2%, 90.7% when hybridized with probes coming from BEP2D, R15H20 and R15H35 cells, respectively. Conclusions The combination of SSH and cDNA microarray is a rapid and effective method for high throughout screening and identification of differentially expressed genes in different samples.The cDNAs confirmed by cDNA microarray may be the relative genes that associate with malignant transformation of bronchial epithelial cells induced by alpha-particles. |
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