| YU Hongsheng,FEI Conghe,SHEN Fangzhen.Effects of low dose radiation on tumor apoptosis,cell cycle progression and changes of apoptosis-related protein bcl-2 in tumor-bearing mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(3):171-173 |
| Effects of low dose radiation on tumor apoptosis,cell cycle progression and changes of apoptosis-related protein bcl-2 in tumor-bearing mice |
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| DOI： |
| KeyWords:Low dose radiation S180 sarcoma Apoptosis Cell cycle bcl-2 |
| FundProject: |
| Author Name | Affiliation | | YU Hongsheng | Department of Oncology, Affiliated Hospital of Qingdao Medical University, Qingdao 266003, China | | FEI Conghe | Department of Oncology, Affiliated Hospital of Qingdao Medical University, Qingdao 266003, China | | SHEN Fangzhen | Department of Oncology, Affiliated Hospital of Qingdao Medical University, Qingdao 266003, China | | 梁军 | Department of Oncology, Affiliated Hospital of Qingdao Medical University, Qingdao 266003, China |
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| Abstract:: |
| Objective To study the effect of low dose radiation (LDR) on tumor apoptosis, cell cycle progression and changes of apoptosis-related protein bcl-2 in tumor-bearing mice. Methods Kunming stain male mice were implanted with S180 sarcoma cells in the left inguen subcutaneously as an in situ experimental animal model.Seven days after implantation, the mice were given 75 mGy whole-body γ-ir radiation.At 24 and 48 h after irradiation, all mice were sacrificed to measure the tumor volume, and tumor cell apoptosis, cell cycle progression were analyzed by flow cytometry.The expression of apoptosis-related protein bcl-2 and the apoptotic rate of tumor cells were observed by immunohistochemistry and electron microscopy. Results Tumor growth was significantly slowed down after LDR(P<0.05).The tumor cells were arrested in G1 phase and the expression of bcl-2 protein decreased at 24 h.Apoptotic rate of tumor cells increased significantly at 48 h after LDR. Conclusion LDR could cause a G1-phase arrest and increase the apoptosis of tumor cells through the low level of apoptosis-related protein bcl-2 in the tumor-bearing mice.The organized immune function and anti-tumor ability are markedly increased after LDR.Our study provides practical evidence of clinical application to cancer treatment. |
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