| QIU Limei,LI Wenjian,FENG Yan,et al.Observation of DNA damage of human hepatoma cells irradiated with heavy ions using comet assay[J].Chinese Journal of Radiological Medicine and Protection,2003,23(3):169-171 |
| Observation of DNA damage of human hepatoma cells irradiated with heavy ions using comet assay |
| Received:May 27, 2002 |
| DOI： |
| KeyWords:SCGE DNA damage SMMC-7721 cell Heavy ions |
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| Author Name | Affiliation | | QIU Limei | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | LI Wenjian | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | FENG Yan | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 高清祥 | 兰州大学 | | 庞新月 | 兰州大学 | | 周利斌 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 毛丽敏 | 兰州大学 | | 陈季军 | 兰州大学 |
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| Abstract:: |
| Objective In this paper, DNA damages induced by irradiation with heavy ions were studied to pile up the base data for clinical therapy. Methods The human hepatoma cells were chosen as the test cell line and were irradiated with 80 MeV/u 20Ne10+, then single cell gel electrophroesis SCGE was used immediately to detect the DNA damages. Results The length of the tails appeared longer as the dose increased, which had the positive correlation with dose;and the number of cells with tail increased along with increasing doses, especially when doses were greater than 2 Gy, in which 100% cells were damage. Conclusion Heavy ions have strong effect to cause DNA damage. |
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