| SUN Xinchen,WANG Junjie,ZHEN Yongsu,et al.Potentiation of radiosensitivity by tetrandrine in human breast cancer cells and its mechanism[J].Chinese Journal of Radiological Medicine and Protection,2003,23(3):160-162 |
| Potentiation of radiosensitivity by tetrandrine in human breast cancer cells and its mechanism |
| Received:April 12, 2002 |
| DOI： |
| KeyWords:Tetrandrine Sensitivity X-ray p53 |
| FundProject:国家杰出青年基金资助项目(30025043);国家自然科学基金资助项目(3947001);美国肿瘤基金会(NFCR)基金资助项目 |
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| Abstract:: |
| Objective To investigate the potentiation of radiosensitivity and mechanism by tetrandrine (Tet) in human breast cancer p53-mutant MCF-7/ADR and p53-wt MCF-7 cells. Methods Clonogenic assay, flow cytometry, Western blotting were preformed in this experiment. Results The data of clonogenic assay showed that Tet markedly sensitized MCF-7/ADR cell to X-rays, and the sensitization enhancement ratio (SER) of Tet was 1 51.Flow cytometry assay showed that exposure of MCF-7/ADR cells to X-rays caused cells to arrest in G2 phase, whereas Tet was able to lower the number of cells arrested in G2 phase.However, in MCF-7 cells, the potentiation effect of Tet was lower, and its SER was 1 10.MCF-7 cells were induced to arrest in G1 and G2 phases by X-rays, and the number of cells arrested in G2 phase abrogated by Tet was less than that in MCF-7/ADR cells.Furthermore, the results showed that the levels of Cyclin B1 and Cdc2 expression decreased after X-irradiation, and the mitotic index was lower too.Tet could reverse this decrease and induce X-ray-irradiated cells to enter mitosis. Conclusion Tet is a potent G2 checkpoint abrogator and markedly enhances the cytotoxicity of X irradiation in the p53-mutant cancer cells. |
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