| MA Shumei,LIU Xiaodong,JU Guizhi.Effect of whole body irradiation with X-rays on cyclinB1,cdc2 gene transcription of bone marrow cells in mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(3):154-156 |
| Effect of whole body irradiation with X-rays on cyclinB1,cdc2 gene transcription of bone marrow cells in mice |
| Received:May 19, 2002 |
| DOI： |
| KeyWords:Ionizing radiation Bone marrow cells CyclinB1 G2 arrest cdc 2 |
| FundProject:国家自然科学基金资助项目(39770193) |
| Author Name | Affiliation | | MA Shumei | MH Radiobiology Research Unit, Norman Bethune Medical Faculty, Jilin University, Changchun 130021, China | | LIU Xiaodong | MH Radiobiology Research Unit, Norman Bethune Medical Faculty, Jilin University, Changchun 130021, China | | JU Guizhi | MH Radiobiology Research Unit, Norman Bethune Medical Faculty, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective For elucidating the molecular mechanism of G2 arrest induced by ionizing radiation, cyclinB1, cdc2 gene transcriptions of bone marrow cells in mice after whole body irradiation(WBI) were studied. Method Semi-quantitative RT-PCR was used to detect cyclinB1, cdc2 mRNA levels, flow cytometry (FCM) was adopted to analyze the cell cycle progression in Kunming mice following WBI with X-rays. Results The data showed an elevation of the percentage of cells in G2 phase at 4 h and 12 h (P<0.05) and an increase of cells in G2+M phase at 12 h after 0.5-6.0 Gy irradiation in a dose-dependent manner.CyclinB1 showed no changes at mRNA level regardless of 2 Gy irradiation in time-course study or 0.5-6.0 Gy in dose-effect study.The cdc2 mRNA level decreased from 4 h, reached its minimum at 12 h (46% of that of sham-irradiation) and began to recover at 48 h.Dose-effect results of cdc2 showed a decrease of cdc2 mRNA level after 0.5-6.0 Gy. Conclusion cdc2 rather than cyclinB1 seems to play important roles in radiation-induced G2 arrest. |
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