| RAN Xinze,SU Yongping,CHENG Tianmin.Effects of abdominal lavage fluid from rats with radiation injury and combined radiation-burn injury on growth of hematopoietic progenitor cells[J].Chinese Journal of Radiological Medicine and Protection,2003,23(3):145-147 |
| Effects of abdominal lavage fluid from rats with radiation injury and combined radiation-burn injury on growth of hematopoietic progenitor cells |
| Received:September 27, 2002 |
| DOI： |
| KeyWords:Radiation injury Combined radiation-burn injury Abdominal lavage fluid Hematopoietic progenitor cells |
| FundProject:国家自然科学基金重点资助项目(30230360,39670289) |
| Author Name | Affiliation | | RAN Xinze | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | SU Yongping | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | CHENG Tianmin | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 郭朝华 | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 刘晓宏 | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 屈纪富 | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 艾国平 | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 林远 | Institute of Comined Injury, PLA, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To observe the effects of abdominal lavage fluid from rats with radiation injury, burn injury and combined radiation-burn injury on growth of hematopoietic progenitor cells. Methods Rats were irradiated with 12 Gy whole body 60Co γ rays and/or inflicted with 30% TBSA III degree burn with a 5 kW bromotungsten lamp.The abdominal lavage fluid from the animals was collected at 3 h, 12 h, 24 h, 48 h, 72 h after injury, separately. Then each of the lavage fluid samples was added to the medium of cultured erythrocyte progenitor cells (CFU-E, BFU-E) or of cultured granulocyte-macrophage progenitor cells (CFU-GM) at a final concentration of 40 μg/ml. Results The colony numbers of CFU-E, BFU-E and CFU-GM formed after addition of the lavage fluid from rats with burn injury or combined radiation-burn injury were significantly higher than those from normal rats at 3 h, 12 h, 24 h, 48 h and 72 h after injury and reached their peak values at 24 h after injury (215.7%, 202.3% and 241.2%, respectively from solely burned rats;188.1%, 147.7% and 204.6%, respectively from rats with combined radiation-burn injury as compared with that of normal rats).However, only few CFU-E, BFU-E or CFU-GM colonies were found after addition of the lavage fluid from solely irradiated rats. Conclusions The abdominal lavage fluid from rats with sole burn injury or combined radiation-burn injury promotes growth of erythrocyte and granulocyte progenitor cells.On the contrary, the lavage fluid from solely irradiated rats shows inhibiting effects. |
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