| KONG Peiyan,LUO Chengji,GUO Chaohua,et al.Effects of expanded bone marrow cells supported by stromal cells on hematopoietic repair in lethally irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(2):89-92 |
| Effects of expanded bone marrow cells supported by stromal cells on hematopoietic repair in lethally irradiated mice |
| Received:May 15, 2002 |
| DOI： |
| KeyWords:Stromal cells Ex vivo expansion Bone marrow mononuclear cells hemotopoietic recovery |
| FundProject:总后卫生部“九·五”指令性攻关课题资助项目(96L045) |
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| Abstract:: |
| Objective To compare the effects of bone marrow cells expanded under different conditions on hematopoietic repair of radiation injuy. Methods In the liquid expanded cultural system with several cytokines and/or a bone marrow stromal cell layer, bone marrow mononuclear cells of mice were expanded for 5 days.Then, the expanded cells were transplanted to lethally irradiated mice via the caudal vein.The hematopoietic recovery of mice after transplantation was assessed by analysing the peripheral blood Hb, WBC, TBC and obser-ving the survival states. Results Ex vivo expansion of bone marrow mononuclear cells with combined use of cytokines under our cultural conditions can not improve the hematopoietic recovery of post-irradiated mice, but the expansion supported by bone marrow stromal cells can accelerate this process significantly. |
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