| LIU Qingjie,CHEN Xiaoning,JIANG Enhai,et al.Establishment of a multicolor FISH method and its application in retrospective dose estimation[J].Chinese Journal of Radiological Medicine and Protection,2003,23(2):77-82 |
| Establishment of a multicolor FISH method and its application in retrospective dose estimation |
| Received:May 21, 2002 |
| DOI： |
| KeyWords:Multicolor FISH Telomere and centromere probes Translocation Previous radiation exposure Dose reconstruction |
| FundProject:卫生部科学研究基金资助项目(98-2-46);美国能源部基金资助项目(DE-FC03-96ER62294) |
| Author Name | Affiliation | | LIU Qingjie | Institute for Radiological Protection and Nuclear Safety, China CDC, Beijing 100088, China | | CHEN Xiaoning | Institute for Radiological Protection and Nuclear Safety, China CDC, Beijing 100088, China | | JIANG Enhai | Cedars Sinai Medical Center, UCLA, USA | | JulieR.Korenberg | Cedars Sinai Medical Center, UCLA, USA | | 姜恩海 | 中国医学科学院放射医学研究所 |
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| Abstract:: |
| Objective To establish an multicolor FISH (M-FISH) method and to investigate its applicability in retrospective dose reconstruction. Methods Bacterial artificial chromosome (BAC) clones specific for telomeres and centromeres of 8 pairs of chromosomes were selected, and an M-FISH method was to be established.Chromosome aberrations in lymphocytes of two new-borns umbilical cord blood having been irradiated with 60Co γ-rays in vitro were analyzed with the M-FISH method.Then the dose-response curves of chromosome aberrations were established.The cumulative doses for two individuals having been exposed to ionizing radiation were estimated with the M-FISH method according to the corresponding dose response curve. Results An M-FISH method, using BAC clones specific for telomeres and centromeres of chromosomes 1, 2, 3, 7, 8, 9, 14 and 15, labeled with biotin-14-dATP and/or digoxigenin-11-dUTP, was established.The 8 pairs of chromosomes could be easily distinguished.The dose-response curve between chromosome aberrations rate and the absorbed dose levels for lymphocytes in umbilical cord blood were of linear-quadratic dose-response model, except for fragments without centromere.The cumulative dose estimated for the two individuals were similar with reciprocal translocation rate in all analyzed cells or in stable cells. Conclusion Dose reconstruction of previous radiation exposure victims can be carried out with the M-FISH method established in this study. |
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