| ZHU Shoupeng,XIAO Dong,WU Yue..Study on apoptosis in HL-60 cells and its related gene regulation induced by enriched uranium[J].Chinese Journal of Radiological Medicine and Protection,2003,23(1):17-19 |
| Study on apoptosis in HL-60 cells and its related gene regulation induced by enriched uranium |
| Received:March 20, 2002 |
| DOI： |
| KeyWords:Enriched uranium 235U HL-60 cells Apoptosis Gene |
| FundProject:国家核工业基金资助项目(Y7197Q6206) |
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| Abstract:: |
| Objective To study characteristics of injury in apoptosis of HL-60 cells and its related genes bcl-2 and bax expression induced by enriched uranium 235U. Methods DNA gel electrophoresis was used to show the apoptosis of HL-60 cells.Immunohistochemical technique was used to identity the expression of bcl-2 and bax proteins in HL-60 cells, meanwhile RNA dot blot hybridization was used to show the expression of bcl-2 mRNA in HL-60 cells. Results HL-60 cells irradiated with 235U displayed characteristics of DNA ladder formation in apoptotic cells.While the expression of bcl-2 protein was as high as (88±7)% in unirradiated control cells, down-regulation [lowered to (61±5)%] of its expression was noted after exposure to 235U.The expression of bax protein was low in control cells, and after irradiation it did not change obviously.A marked down-regulation of bcl-2 mRNA expression was found in irradiated HL-60 cells. Conclusion Progression of apoptosis in HL-60 cells induced by 235U is dependent on the time elapse of 235U irradiation.The apoptotic action of 235U in HL-60 cells is associated with down-regulation of the apoptosis-related gene bcl-2. |
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