| MENG Qingyong,CHEN Shali,LIU Shuzheng.Effect of low dose radiation on thymocyte cytosol and nuclei protein synthesis in mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(1):14-16 |
| Effect of low dose radiation on thymocyte cytosol and nuclei protein synthesis in mice |
| Received:April 26, 2002 |
| DOI： |
| KeyWords:Low dose radiation Thymocyte cytosol Thymocyte nuclei Protein expression High performance liquid chromatography |
| FundProject:国家自然科学基金资助项目(39570188) |
|
| Hits: 2850 |
| Download times: 1731 |
| Abstract:: |
| Objective To the effect of low dose radiation on thymocyte cytosol and nuclei protein synthesis in mice. Methods The expression of proteins was analyzed by gel filtration with Sephadex G-100 and HPLC based on separation of proteins on thymocyte cytosol and nuclei after whole-body irradiation with 75 mGy X-rays and sham-irradiation, and their biological activity was examined by mouse splenocyte proliferation and chromosome aberration of human peripheral blood lymphocytes. Results HPLC analysis showed that there was a marked increase in expression of 61.4 kD protein in the extract of thymocyte cytosol and 30.4 kD protein in the extract of thymocyte nuclei in comparison with the corresponding fractions from the sham-irradiated control mice.These protein fractions from the thymocyte cytosol and nuclei of the irradiated mice showed both stimulating effect on normal T cell proliferation and protective effect on chromosome damage induced by high dose radiation. Conclusion These findings might have implications in study of mechanism of immunoenhancement and cytogenetic adaptive response induced by low dose radiation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
