| LIU Xiao dong,MA Shumei,LIU Shuzheng..Effects of ionizing radiation on IL-12 and NF-κB activities in peritoneal macrophages of mice[J].Chinese Journal of Radiological Medicine and Protection,2003,23(1):7-10 |
| Effects of ionizing radiation on IL-12 and NF-κB activities in peritoneal macrophages of mice |
| Received:March 26, 2002 |
| DOI： |
| KeyWords:Ionizing radiation IL-12 NF -κB ELISA EMSA |
| FundProject:国家自然科学基金资助项目(39570188) |
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| Abstract:: |
| Objective To observe the changes of IL-12 and NF -κB in murine peritoneal macrophages after whole-body irradiation(WBI) with different doses X-rays. Methods Enzyme-linked immunosorbent assay(ELISA) and electrophoresis mobility shift assay(EMSA) were used to detect the secretion of IL-12 and the DNA-binding activity of NF -κB, respectively. Results ELISA data indicated both 0 075 Gy and 2 Gy increased the secretion of IL-12 and the latter seemed to increase IL-12 more greater than the former.EMSA showed two bands representing P65/P50 and P50/P50 respectively. 0 075 Gy increased the P65/P50 activity and showed little effect on P50/P50, while 2 Gy increased both the two dimmers, consequently elevated the DNA-binding activity of NF -κB more significantly. Conclusion The results suggested that NF -κB might participate in the radiation-induced changes of IL-12. |
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