| JU Guizhi,WANG Xiaomei,FU Shibo.Effect of ionizing radiation on p16 gene transcription and protein expression[J].Chinese Journal of Radiological Medicine and Protection,2003,23(1):4-6 |
| Effect of ionizing radiation on p16 gene transcription and protein expression |
| Received:June 03, 2002 |
| DOI： |
| KeyWords:Ionizing radiation p16mRNA p16 protein expression |
| FundProject:国家自然科学基金资助项目(39770193) |
| Author Name | Affiliation | | JU Guizhi | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | WANG Xiaomei | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | FU Shibo | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | 刘树铮 | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To investigate the effect of ionizing radiation on p16 gene transcription and protein expression. Methods Northern blot was employed to determine p16mRNA level. Flowcytometry was used to measure p16 protein expression. Results It was demonstrated that p16mRNA level was markedly increased at 2-24 h in thymocytes and at 2-8 h in splenocytes, and p16 protein expression increased significantly at 8-48 h for thymocytes ( P<0.05 to P<0.01) and at 24 h for splenocytes ( P< 0.05) after whole body X-irradiation with 2.0 Gy in mice.In the dose-effect experiments, it was found that p16mRNA level increased with the increase of dose in thymocytes and splenocytes and the p16 protein expression was also increased significantly with the doses from 1.0 to 4.0 Gy for both thymocytes and splenocytes (P<0.05 to P<0.01). Conclusion p16 gene transcription and portein expression could be up-regulated by X-rays and the tendency of increase varied with the radiation dose and cell type. |
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