| LI Hua,LIU Wen,LI Jinyan,et al.Effection of low dose ionizing radiation on expression of DNA topoisomerase Ⅱα[J].Chinese Journal of Radiological Medicine and Protection,2003,23(1):1-3 |
| Effection of low dose ionizing radiation on expression of DNA topoisomerase Ⅱα |
| Received:March 21, 2002 |
| DOI： |
| KeyWords:Low dose ionizing radiation Topoisomerase Ⅱα Gene expression |
| FundProject:国家自然科学基金资助项目(30100037) |
| Author Name | Affiliation | | LI Hua | Department of Cellular and Genetic Medicine, School of Medicine, Fudan University, Shanghai 200032, China | | LIU Wen | 200032 复旦大学医学院细胞与遗传医学系 | | LI Jinyan | Department of Cellular and Genetic Medicine, School of Medicine, Fudan University, Shanghai 200032, China | | 左伋 | Department of Cellular and Genetic Medicine, School of Medicine, Fudan University, Shanghai 200032, China |
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| Abstract:: |
| Objective To explore the changes of topoisomerase (TOPO) Ⅱα expression, the behavior of TOPO Ⅱα in HeLa cells treated with low dose ionizing radiation was studied. Methods The kinetics of TOPO Ⅱα was measured by immunocytochemistry and Western blot. Results A profound and transient decrease of TOPOⅡα after γ irradiation with 5 Gy was detected, the decrease began from 4 h after irradiation, reached the lowest level at 12 h, and began to return at 16 h.Given low dose γ-rays, the level decreased along with the increase of dose.The level of decrease after several times irradiation was higher than that after only one time. Conclusions This observation indicates that the decrease of TOPO Ⅱα after γ-irradiation is time-dependent and dose-dependent, and the effect of low dose γ irradiation can be accumulated. |
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