| ZHANG Junning,HONG Chengjiao,ZHU Shoupeng.The apoptosis and expression of Bcl-2 mRNA,Bcl-2 and Bax of K562 cells following internal exposure to strontium-89[J].Chinese Journal of Radiological Medicine and Protection,2002,22(6):416-418 |
| The apoptosis and expression of Bcl-2 mRNA,Bcl-2 and Bax of K562 cells following internal exposure to strontium-89 |
| Received:November 12, 2001 |
| DOI： |
| KeyWords:Strontium-89 Internal exposure K562 cells Apoptosis bcl-2 bax bcl-2 mRNA |
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| Abstract:: |
| Objective To explore the characteristics and mechanism of apoptosis in K562 cells induced by radianuclides strontium-89. Methods Using molecular and quantitative pathological technique,the apoptosis and expressions of bcl-2 mRNA,bcl-2 and bax protein of K562 cells following internal exposure to strontium-89 were studied. Results At 6 hrs after irradiation,the characteristic featares of apoptosis and necrosis appeared in K562 cells.The apoptosis and necrosis were enhanced with the prolongation of internal contamination time from 6,9,12,24 to 48 hrs.The expression of bcl-2 mRNA decreased at 12 hrs,most remarkably at 24 hrs,The expression of bcl-2 protein decreased after irradiation whereas bax protein did not change obviously. Conclusion The results suggest that apoptosis induced by internal exposure may be regulated by reducing expressions of bcl-2 mRNA,bcl-2 protein,and lowering bcl-2/bax ratio. ; |
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