| SUN Jingfen,SUI Jianli,GENG Yu,et al.The studies of DNA double-strand break (DSB) rejoining and mRNA expression of repair gene XRCCs in malignant transformed cell lines of human bronchial epithelial cells generated by α-particles[J].Chinese Journal of Radiological Medicine and Protection,2002,22(6):405-408 |
| The studies of DNA double-strand break (DSB) rejoining and mRNA expression of repair gene XRCCs in malignant transformed cell lines of human bronchial epithelial cells generated by α-particles |
| Received:December 15, 2001 |
| DOI： |
| KeyWords:Human bronchial epithelial cells α-particles DNA repair gene mRNA expression Carcinogenesis |
| FundProject:“十五”军队医学杰出中青年基金资助项目(01J006);国家高技术研究发展863计划资助项目(Z001AA221271) |
| Author Name | Affiliation | | SUN Jingfen | Beijing Institute of Radiation Medicine, Beijing 100850, China | | SUI Jianli | Beijing Institute of Radiation Medicine, Beijing 100850, China | | GENG Yu | Beijing Institute of Radiation Medicine, Beijing 100850, China | | 周平坤 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | 吴德昌 | Beijing Institute of Radiation Medicine, Beijing 100850, China |
|
| Hits: 3763 |
| Download times: 2354 |
| Abstract:: |
| Objective To investigate the efficiency of γ-ray-induced DNA DSB rejoining and the mRNA expression of DNA repair genes in malignantly transformed cell lines of human bronchial epithelial cells generated by exposure to α-particles. Methods Pulsed field gel electrophoresis (PFGE) was used to detect DNA.DSBs mRNA expression was analyzed by RT-PCR. Results The residual DNA DSB damage level after 4hrs repair following 0-150 Gy of γ-irradiation in the malignantly transformed cell lines BERP35T-1 and BERP35T-4 was significantly higher than that in their parental BEP2D cells.The analysis of mRNA level revealed a 2.5-to 6.5-fold down-regulated expression of the DNA repair genes XRCC-2,XRCC-3 and Ku80(XRCC-5) in BERP35T-1 and BERP35T-4 cells as compared with the parental BEP2D cells.In contrast,the expression of DNA-PKcs(XRCC7) was 2.4-fold up-regulated in the transformed cell line BERP35T-4,in which there was a significantly higher pro-portion of polyloid cells. Conclusion This study results show that the deficiency of DNA DSB rejoining and depressed mRNA expression of DNA repair genes could be involved in the malignant transformation process of BEP2D cells induced by exposure to α-particles. ; |
| HTML View Full Text View/Add Comment Download reader |
| Close |
