QIN Quanhong,WANG Dewen,PENG Ruiyun,et al.Expression of PDGF-A and PDGFR-α in fibroblasts of usual and radiation-combined wound healing[J].Chinese Journal of Radiological Medicine and Protection,2002,22(6):401-404
Expression of PDGF-A and PDGFR-α in fibroblasts of usual and radiation-combined wound healing
Received:September 19, 2001  
DOI:
KeyWords:Irradiation  Wound healing  PDGF-A  PDGFR-α
FundProject:全军重点招标课题资助项目(96Z008)
Author NameAffiliation
QIN Quanhong Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
WANG Dewen Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
PENG Ruiyun Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
谷庆阳 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
高亚兵 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
夏国伟 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
崔雪梅 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
刘杰 Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 
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Abstract::
      Objective To study the impact of local γ-irradiation on expression of PDGF-A and PDGFR-α in fibroblasts during healing process of usual and radiation-combined wounds. Methods 132 Wistar rats were divided into two groups:usual wound group (control group, n =66) and radiation-combined wound group (radiation group, n =66).Immunohistochemistry, in situ RT-PCR and image analysis were performed to detect the expression of PDGF-A,PDGFR-α and PDGF-A mRNA transcription in these two groups. Results Every stage of healing was delayed by radiation.The expressions of PDGF-A and PDGFR-α in the control group had temporal characteristic:PDGF-A expression was observed on days 2-21 and the highest expression was on day 5. PDGFR-α was detected on days 2-15 and its highest expression was on day 5.In the radiation group the expressions of PDGF-A were weaker than those in the control group and the expression time was prolonged,it was still observed on day 28,whereas the expression of PDGFR-α was weaker than that in the control group.But its temporal characteristic was the same as that in the control group. Conclusion Radiation can reduce the expression of PDGF-A and PDGFR-α in wound healing,indicating that it inhibits the secretion on endogenous PDGF-A and reduces the reactivity of wound to exogenous PDGF-A,thus wound healing is delayed.
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