QIN Quanhong,WANG Dewen,PENG Ruiyun,et al.Expression of PDGF-A and PDGFR-α in fibroblasts of usual and radiation-combined wound healing[J].Chinese Journal of Radiological Medicine and Protection,2002,22(6):401-404 |
Expression of PDGF-A and PDGFR-α in fibroblasts of usual and radiation-combined wound healing |
Received:September 19, 2001 |
DOI: |
KeyWords:Irradiation Wound healing PDGF-A PDGFR-α |
FundProject:全军重点招标课题资助项目(96Z008) |
Author Name | Affiliation | QIN Quanhong | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | WANG Dewen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | PENG Ruiyun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | 谷庆阳 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | 高亚兵 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | 夏国伟 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | 崔雪梅 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 | 刘杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850 |
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Abstract:: |
Objective To study the impact of local γ-irradiation on expression of PDGF-A and PDGFR-α in fibroblasts during healing process of usual and radiation-combined wounds. Methods 132 Wistar rats were divided into two groups:usual wound group (control group, n =66) and radiation-combined wound group (radiation group, n =66).Immunohistochemistry, in situ RT-PCR and image analysis were performed to detect the expression of PDGF-A,PDGFR-α and PDGF-A mRNA transcription in these two groups. Results Every stage of healing was delayed by radiation.The expressions of PDGF-A and PDGFR-α in the control group had temporal characteristic:PDGF-A expression was observed on days 2-21 and the highest expression was on day 5. PDGFR-α was detected on days 2-15 and its highest expression was on day 5.In the radiation group the expressions of PDGF-A were weaker than those in the control group and the expression time was prolonged,it was still observed on day 28,whereas the expression of PDGFR-α was weaker than that in the control group.But its temporal characteristic was the same as that in the control group. Conclusion Radiation can reduce the expression of PDGF-A and PDGFR-α in wound healing,indicating that it inhibits the secretion on endogenous PDGF-A and reduces the reactivity of wound to exogenous PDGF-A,thus wound healing is delayed. |
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