| YANG Guang,LIU Chang-an,JIA Tingzhen,et al.Effect of low dose radiation on immune function of cord blood cells:A preliminary investigation[J].Chinese Journal of Radiological Medicine and Protection,2002,22(6):394-396 |
| Effect of low dose radiation on immune function of cord blood cells:A preliminary investigation |
| Received:December 11, 2001 |
| DOI： |
| KeyWords:Low dose irradiation Cord blood Natural killer cell Lymphocytes |
| FundProject:国家自然科学基金资助项目(39770238) |
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| Abstract:: |
| Objective To investigate the effects of low dose radiation (LDR) on lymphocyte proliferation,CD25 expression,interleukin-2 (IL-2) level,and natural killer cell(NK cell) activity in cord blood. Methods (1)Isolated mononuclear cells from cord blood were irradiatied with different doses (62 mGy,124 mGy,186 mGy) of gamma-rays.The lymphocyte proliferation and NK cell activity were measured with 3H-TdR incorpation assay and 3H-TdR release assay,respectively.(2)Isolated fresh fresh mononuclear cells from cord blood were irradiated with 62 mGy gamma-rays.At different culture times (12 h,24 h,48 h) after irradiation,CD 25 expression was measured by flow cytometry with direct immunofluoresence and IL-2 levels in the supernatant was measured by ELISA. Results As compared with controls,LDR could promote lymphocyte proliferation and enhance NK cell activity.The CD25 expression and the IL-2 level in cord blood were up-regulated significantly after 62 mGy gamma-ray irradiation and showed increasing amplitude with time. Conclusion LDR may promote proliferation,maturity and activation of lymphocyte and enhance NK cell activity in cord blood,which may contribute to the enhanced graft versus leukemia(GVL) response and the decrease of the recurrence rate of tumor,as well as acceleration of the reconstruction of immune and hematopietic functions after cord blood transplantation,using low dose γ-irradiated mononuclear cells from cord blood is of value in clinical practice. ; |
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