| SHI Jianhui,XU Xiaoping,CHENG Wenying,et al.Inhibitory effects of glucocorticoid on apoptosis and activation of NF-κB in P388 cells induced by radiation[J].Chinese Journal of Radiological Medicine and Protection,2002,22(6):391-393 |
| Inhibitory effects of glucocorticoid on apoptosis and activation of NF-κB in P388 cells induced by radiation |
| Received:January 19, 2002 |
| DOI： |
| KeyWords:Radiation P388 NF-κB Apoptosis |
| FundProject:国家自然科学基金资助项目(39770330) |
| Author Name | Affiliation | | SHI Jianhui | Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | XU Xiaoping | 第二军医大学长海医院血液科 | | CHENG Wenying | 复旦大学放射医学研究所 | | 李林 | | | 冯晓 | 复旦大学放射医学研究所 | | 张宗梁 | 中国科学院上海细胞生物研究所分子细胞生物学开放实验室 | | 牛玉宏 | Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | 葛均波 | Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai 200032, China |
|
| Hits: 3645 |
| Download times: 2423 |
| Abstract:: |
| Objective To explore effects of glucocorticoid on apoptosis and activation of NF-κB in P388 cells induced by radiation. Methods Apoptosis in P388 cells induced by radiation treatment was detected by TUNEL assay.EMSA was used to detect the activation of NF-κB. Results The apoptosis and activation of NF-κB in P388 cells could be induced by radiation.Dexamethasone (DXM) which could suppress activation of NF-κB of P388 cells increased significantly the apoptosis induced by radiation.Apoptosic rates in DXM-treated P388 cells after 2,4,6 and 8 Gy exposure increased by 60%,100%,129% and 67%,respectively.Activation rates of NF-κB in DXM-treated P388 cells after 2,4,6,and 8 Gy exposure decreased by 25%,45%,52% and 40%,respectively. Conclusion Radiation induces apoptosis and activation of NF-κB in P388 cells simultaneously.Glucocorticoid enhances apoptosis in leukemic cells,which may be by means of suppressing activation of NF-κB. ; |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
