| LIU Guangwei,LI Pengwu,LIU Shuchun.Adaptive response of spermatogenic cell apoptosis induced by low dose X-ray irradiation in mice[J].Chinese Journal of Radiological Medicine and Protection,2002,22(5):322-325 |
| Adaptive response of spermatogenic cell apoptosis induced by low dose X-ray irradiation in mice |
| Received:October 12, 2001 |
| DOI： |
| KeyWords:Lowdose irradiation Adaptive response Spoptosis Spermatogenic cell TUNEL |
| FundProject: |
| Author Name | Affiliation | | LIU Guangwei | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | LI Pengwu | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | LIU Shuchun | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China | | 龚守良 | MH Radiobiology Research Unit, Jilin University, Changchun 130021, China |
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| Abstract:: |
| Objective To study the adaptive response of spermatogenic cell apoptosis induced by whole body X ray irradiation with low doses in male Kunming mice. Methods The spermatogenic cell apoptosis induced by 75 mGy X ray irradiation during spermatogenic cell cycle was measured by using the methods of terminal deoxyribonucleotide transferase mediated dUTP bictin nick end labeling (TUNEL) and HE staining. Results When an inductive dose (D1) of 75 mGy was given or not at 6 h before the challenging dose (D2) of 1.0,2.0 or 3.0 Gy,the apoptotic percentages of the spermatogonia and spermatocytes in the D1+D2 groups were declined rapidly as compared with those in the D2 alone groups,and the apoptotic percentages of spermatids showed no significant changes.When the inductive dose (D1) 75 mGy was given at 3,6,12 and 24 h before the challenging doses (D2) of 1.0,1.5,2.0,2.5 and 3.0 Gy,the apoptotic percentages in spermatogonia and spermatocytes reduced early and significantly and continued for a longer duration under smaller D2 doses (1.0,1.5 and 2.0 Gy).However,the apoptotic percentages reduced only at 6 h after whole body irradiation under larger D2 doses (2.5 and 3.0 Gy),and were not significant. Conclusion sThe adaptive response of apoptosis in spermatogonia and spermatocytes could be induced by low doses X ray irradiation.The effect could closely relate to D2 doses and the interval time between D1 and D2. |
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