| YANG Zhenguo,WANG Dewen,SONG Liangwen.Dose-effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts[J].Chinese Journal of Radiological Medicine and Protection,2002,22(5):311-314 |
| Dose-effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts |
| Received:November 15, 2001 |
| DOI： |
| KeyWords:Nitric oxide Human embryo lung fibroblast 60 Coγray Proliferation |
| FundProject:国家自然科学基金资助项目(29807213) |
| Author Name | Affiliation | | YANG Zhenguo | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | WANG Dewen | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | SONG Liangwen | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | 彭瑞云 | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | 秦全红 | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | 夏国伟 | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | 李杨 | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | 高亚兵 | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China | | 崔雪梅 | Institute of Radiation Medicine Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To investigate the dose effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts. Methods MTT colorimetry and immunohistochemistry were used to observe the proliferative activity and expression of ET.TGFβ.AⅡ in fibroblasts. Results Low dose 60 Co γ-ray promoted proliferation of cultured HELF cells especially 5 Gy.NO provider sodium nitroprusside released NO linearly within 30 hours by measuring the concentration of NO in the culture medium.The average increase amplitude was 4.25 μmol/L.The optimum concertration to depress hyperplasia of fibroblast was 4～5 μmol/L.The ET、TGFmRNA transcription and protein expression of ET、TGF.AⅡ were increased after irradiation. Conclusion NO can inhibit excessive proliferation and secretion of fibroblasts in a dose dependent way. |
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